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CABRI:DSMZ_PLASMID |
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Collection_number
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DSM 3879
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Name |
pBR322
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Other_culture_collection_numbers |
-
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Type |
plasmid
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Class |
recombinant
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Constructed_from |
pMB1, pSC101 and Tn3
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Literature |
DSM ref.no. 3138; DSM ref.no. 3795
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History_of_deposit |
<- M. Reh
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Restricted_distribution |
yes: GEM (C)
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Host_for_distribution |
Escherichia coli K12 SK1592
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Medium |
381, 37C
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Selectable_phenotype |
Ampicillin resistance, Tetracycline resistance
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Replicon |
pMB1
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Transfer_ability |
non-mobilizable
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Host_range |
Escherichia coli vector
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Properties_and_applications |
General cloning vector; Expression vector: general expression
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Copy_number |
15-20
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Molecular_weight |
4.36 kb
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Cloned_gene |
Apr, Tcr
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Further_information |
Constructed plasmid; versatile cloning vector; unique restriction sites: PstI;
SalI, EcoRI, HindIII, BamHI; insertional inactivation of Ap and Tc genes.
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Restriction_sites |
(U,I): BamHI(tetr), PstI(ampr), SalI(tetr); (U): EcoRI, HindIII
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